Cell sorters
Please find below the detailed information on our sorters. The linked file will help you make a decision on which sorter to book for your experiment.
BD FACSDiscover™ S8 Cell Sorter
The BD FACSDiscover™ S8 Cell Sorter is a spectral instrument equipped with 5 lasers and 78 detectors. Furthermore, its BD CellView™ Image Technology allows for image acquisition from 3 fluorescence channels (488 nm excitation), forward scatter, side scatter and axial light loss. The real-time integration of image and flow cytometry data provides a possibilit to visualize and confirm cell types in real time before cell sorting.
The available nozzles sizes are 85 μm (35 psi), 100 μm( 20 psi) and 130 μm (7psi).
Up to 6-way sorting for all nozzles is possible. Sort collection devices include:
Tube sorting: 15-mL microtubes, 2.0-mL and 12x75-mm (5.0-mL) tubes, 15-mL and 50-mL centrifuge tubes
Plate sorting: 6-, 24-, 48-, 96- and 384- well plates, microscope slide (optional)
InvitrogenTM Bigfoot Spectral Cell Sorter
The Bigfoot jet-in-air spectral cell sorter is equipped with 7 lasers : 349nm, 405nm, 445nm, 488nm, 561nm, 640nm and 785 nm and 60 detectors. It allows acquisition and sorting on spectrally unmixed or coventionally compensated data.
The available nozzles include 70 μm, 100 μm , 120 μm , 150 μm and 200 μm nozzles.
With sort rates up to > 70 000 eps, sorting is fast and configurable. Possible collections media include 1.5 ml. 5 ml, 15 ml, 50 ml tubes , 96- well plates (four-way sorting), 394-well plates (eight-way sorting) , 1536- well plates (straight-down sorting), as well as 96- and 394- deep well plates. 96- well sort can take as little as 11 seconds and 384-well sort 20 seconds. Futhermore, micoroscopic slides, 8 well strips and 10 x Genomics chips can be used as collection devices.
Sasquatch (SQ) software manages acquisition, analysis, and sorting. An advanced fluorophore selector helps to design standard and spectral experiments and warns of potential conflicts. The experiment wizard assists in running controls, identifying potential issues, and applying the unmixing algorithms. Analysis features include overlays, color-gating, backgating, index sorting, and ratio plots, and the software generates FCS files.
BD FACSAriaIII - BSL1 sorter
High-speed cell sorter with a fixed-alignment cuvette flow cell
4 laser paths: nearUV (375nm)/violet (405nm), blue (488nm), yellow-green (561nm) and red (633nm)
Multicolor analysis of up to 16 parameters and sorting rates of up to 25,000 events/sec are possible
70μm, 85μm, 100μm and 130μm nozzles are available
Up to 4-way sorting into 1.5ml or 5ml tubes, 2-way sorting into 15ml tubes Single cell sorting in 6-, 24-, 96-and 384-well plates
BD FACSAria Fusion - BSL2 sorter
The BD FACSAriaTM Fusion Cell Sorter is placed in the biosafety hood for increased operator and sample protection.
BD Aria Fusion is equipped with 5 lasers (355, 405, 488, 561 and 633 nm) and can measure up to 20 parameters.
It is a high speed-cell sorter with a sensitive, fixed-alignment couvette flow cell.
The 70, 85, 100 and 130-micron nozzle tip sizes are available to accomodate a broad range of cell types.
Two- and four-way bulk sorting devices for a variety of tube sizes are provided.
Automated Cell Deposition Unit allows plate and slide sorting. Index sorting makes it possible to review the complete cell surface phenotype of every cell single sorted into plates. BD FACSDiva software manages acquisition templates, experiment layouts, and compensation procedures.